A stability-indicating
reverse phase ultra-performance liquid chromatography (RP-UPLC) method,
developed for the simultaneous quantification of eight related compounds in the
dosage forms of Emtricitabine, Tenofovir Disoproxil Fumarate and Efavirenz.
Efficient
chromatographic separation was achieved on an Acquity UPLC BEH Phenyl (2.1×100
mm, 1.7 m) column using mobile phase A (Buffer, 1.0 mL of TEA in 1000 mL of
water pH adjusted to 4.0 ± 0.05 with diluted OPA) and mobile phase B in
gradient mode with the flow rate of 0.5 mL/min and the peaks were monitored at
265 nm.
In the developed
method, the resolution of Emtricitabine, Tenofovir Disoproxil Fumarate,
Efavirenz and related substances were found to be greater than 2.0.
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